For thoses that want clarification. Endotoxins are toxins that are present on the surface of bacteria. On ingestion it provoke an immune response like fever or likely an anaphylactic choc that can lead to death.

Just to add a bit of practical information (in case somebody desperate is reading this in five years), they’re very hard to separate from other things and tend to stick around largely invisibly. Very small amounts can provoke deadly immune responses. Labs who do make endotoxin free samples often have entirely separate sets of equipment for endotoxin free preps.

What we really need for companies or non-profits to take the expired patent versions of these drugs and produces them cheaply and safely.

Please google endotoxins before attempting this. It’s really, really important to work with pure and sterilized samples.

Yeah, I had seen the OT-2 before. I’m not sure it quite reaches the standard I’m looking for, but it might be the best I can hope for. The user manual seems to document a lot even if I’d like a more details on a lot of things. I’m certainly not paying 3k for a pipette and a subscription to over priced tips, but maybe someone has documented some mix of casting and 3D printing that to make a more sensible pipette. Ideally, we’d have open blueprints and repair manuals, but maybe there’s enough of a community around it to make it the best starting place.